This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3.1-IDH2-WT
catalog :
87926
citations: 1
Reference
Showalter M, Hatakeyama J, Cajka T, VanderVorst K, Carraway K, Fiehn O. Replication Study: The common feature of leukemia-associated IDH1 and IDH2 mutations is a neomorphic enzyme activity converting alpha-ketoglutarate to 2-hydroxyglutarate. elife. 2017;6: pubmed publisher
product information
Catalog Number :
87926
Product Name :
pcDNA3.1-IDH2-WT
article :
doi10.7554/eLife.26030
id28189888
pubmed_id28653623
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.1
backbone_mutation
backbone_originInvitrogen, cat# V790-20
backbone_size5428
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Expresses IDH2 (wild-type) in mammalian cells
origin :
37
pi :
alt_names
IDH2
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5BamH1
promoterCMV
sequencing_primer_3BGH Reverse
sequencing_primer_5T7 Forward
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesD2HGA2, ICD-M, IDH, IDH-2, IDHM, IDP, IDPM, mNADP-IDH
geneIDH2
id3418
genbank_ids
NM_002168.3
mutation
nameisocitrate dehydrogenase (NADP(+)) 2, mitochondrial
shRNA_sequence
size1359
species
9606
Homo sapiens
tags
location
tagnone
plasmid copy :
IDH2 WT was amplified by PCR from pCMV6-Entry vectors (Origene, cat# RC201152)
resistance markers :
3441
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA