This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCMV-hATG7CS
catalog :
87868
citations: 1
Reference
Tanida I, Tanida Miyake E, Ueno T, Kominami E. The human homolog of Saccharomyces cerevisiae Apg7p is a Protein-activating enzyme for multiple substrates including human Apg12p, GATE-16, GABARAP, and MAP-LC3. J Biol Chem. 2001;276:1701-6 pubmed
product information
Catalog Number :
87868
Product Name :
pCMV-hATG7CS
article :
doi10.1074/jbc.C000752200
id25065
pubmed_id11096062
bacterial resistance :
Kanamycin
cloning :
backbonepEGFP-N1
backbone_mutation
backbone_origin
backbone_size4000
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Based on pCMV-hATG7wt, an active site Cys572 within human ATG7 was changed to Ser by site-directed mutagenesis. An unlinked, promoterless, EGFP is also present in the backbone of the plasmid.
growth strain :
Express a active-site mutant human Atg7/Apg7 C572S in mammalian cells
origin :
37
pi :
alt_names
autophagy-related gene 7 C572S mutant
APG7 C572S mutant
ATG7 active site-mutant
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3
sequencing_primer_5ATGTCGTAACAACTCCGCC
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAPG7-LIKE, APG7L, GSA7, SCAR31
geneATG7
id10533
genbank_ids
AF094516
mutationC572S mutant
nameATG7 C572S mutant
shRNA_sequence
size2123
species
9606
Homo sapiens
tags
resistance markers :
4512
739
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA