This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pEGFPN1-N-gamma-tubulin (334-449)
catalog :
87859
citations: 2
Reference
Alvarado Kristensson M. A simple and fast method for fixation of cultured cell lines that preserves cellular structures containing gamma-tubulin. MethodsX. 2018;5:227-233 pubmed publisher
Höög G, Zarrizi R, von Stedingk K, Jönsson K, Alvarado Kristensson M. Nuclear localization of ?-tubulin affects E2F transcriptional activity and S-phase progression. FASEB J. 2011;25:3815-27 pubmed publisher
product information
Catalog Number :
87859
Product Name :
pEGFPN1-N-gamma-tubulin (334-449)
article :
doi10.1096/fj.11-187484
id11226
pubmed_id21788450
bacterial resistance :
Kanamycin
cloning :
backbonepEGFP-N1
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
The C-terminal is a sh-gamma-tubulin resistant gene. C 234 and 240 are mutated to T
growth strain :
Fluorescent fragment of gamma-tubulin containing gamma-tubulin DNA binding domain and NLS
origin :
37
pi :
alt_names
TUBG1
cloning
entrez_gene
aliasesCDCBM4, GCP-1, TUBG, TUBGCP1
geneTUBG1
id7283
genbank_ids
NM_001070.4
mutationfragment from aa 334 to 449
namegamma-tubulin 1
shRNA_sequence
size345
species
9606
Homo sapiens
tags
locationC terminal on insert
tagGFP
plasmid copy :
The TUBG1 was originally cloned by Dr. Jiri Bartek, I subclonned the c-terminal region into the pEGFN1 vector.
resistance markers :
2423
tags :
Unknown
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA