This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCR2.1_HBB_A
catalog :
87848
citations: 1
Reference
Patsali P, Papasavva P, Christou S, Sitarou M, Antoniou M, Lederer C, et al. Relative and Absolute Quantification of Aberrant and Normal Splice Variants in HBBIVSI-110 (G > A) β-Thalassemia. Int J Mol Sci. 2020;21: pubmed publisher
product information
Catalog Number :
87848
Product Name :
pCR2.1_HBB_A
article :
doi10.3390/ijms21186671
id25056
pubmed_id32933098
bacterial resistance :
Ampicillin and Kanamycin
cloning :
backbonepCR2.1-TOPO
backbone_mutation
backbone_originInvitrogen (Life Technologies)
backbone_size3930
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth strain :
pCR2.1-based plasmid holding a 328-bp fragment containing the exon-1-exon-2 splice border of aberrant HBB[IVSI-110(G>A)] human -globin mRNA
origin :
37
pi :
alt_names
cloning
clone_methodTOPO Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3M13 Forward Primer
sequencing_primer_5M13 Reverse Primer
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCD113t-C, ECYT6, beta-globin
geneHBB
id3043
genbank_ids
NM_000518.4
mutationHBB(IVSI-110) -thalassaemia misplicing mutation (HGVS name: HBB:c.93-21G>A)
nameHomo sapiens hemoglobin subunit beta (HBB), Aberrant cDNA fragment (Exon1/19nt mispliced Intron1 + Exon2)
shRNA_sequence
size329
species
9606
Homo sapiens
tags
plasmid copy :
Petros Patsali
resistance markers :
5041
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA