This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
TLCV2-RB1
catalog :
87836
citations: 3
Reference
Han W, Liu M, Han D, Toure A, Li M, Besschetnova A, et al. Exploiting the tumor-suppressive activity of the androgen receptor by CDK4/6 inhibition in castration-resistant prostate cancer. Mol Ther. 2022;30:1628-1644 pubmed publisher
D Oto A, Fang J, Jin H, Xu B, Singh S, Mullasseril A, et al. KDM6B promotes activation of the oncogenic CDK4/6-pRB-E2F pathway by maintaining enhancer activity in MYCN-amplified neuroblastoma. Nat Commun. 2021;12:7204 pubmed publisher
Barger C, Branick C, Chee L, Karpf A. Pan-Cancer Analyses Reveal Genomic Features of FOXM1 Overexpression in Cancer. Cancers (Basel). 2019;11: pubmed publisher
product information
Catalog Number :
87836
Product Name :
TLCV2-RB1
article :
doi10.3390/cancers11020251
id24988
pubmed_id30795624
bacterial resistance :
Ampicillin
cloning :
backboneTLCV2
backbone_mutation
backbone_origin
backbone_size
promoterTight TRE promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
CRISPR
Other
Doxycycline inducible; eGFP reporter
growth notes :
The sgRNA targeting RB1 (sgRB#1) was previously published - Nicolay BN, et al. Proteomic analysis of pRb loss highlights a signature of decreased mitochondrial oxidative phosphorylation. Genes Dev. 2015;29(17):1875 1889. The oligos corresponding to sgRB#1 were ordered then annealed and ligated into TLCV2.
growth strain :
LentiCRISPR v2 was modified into an all-in-one dox inducible system. The addition of doxycycline induces Cas9-2A-eGFP. The U6 promoter drives constitutive expression of an sgRNA targeting human RB1.
origin :
37
pi :
alt_names
RB1, RB, RB Transcriptional Corepressor 1
cloning
clone_methodRestriction Enzyme
cloning_site_3BsmBI
cloning_site_5BsmBI
promoterU6
sequencing_primer_3
sequencing_primer_5human U6-F GAGGGCCTATTTCCCATGATT
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesOSRC, PPP1R130, RB, p105-Rb, p110-RB1, pRb, pp110
geneRB1
id5925
genbank_ids
NM_000321
mutation
namesgRB1
shRNA_sequenceGCTCTGGGTCCTCCTCAGGA
size
species
9606
Homo sapiens
tags
resistance markers :
2517
tags :
Unknown
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA