This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCA24N-ligase-pprA
catalog :
87744
citations: 1
Reference
Wilson R, Morton S, Deiderick H, Gerth M, Paul H, Gerber I, et al. Engineered DNA ligases with improved activities in vitro. Protein Eng Des Sel. 2013;26:471-8 pubmed publisher
product information
Catalog Number :
87744
Product Name :
pCA24N-ligase-pprA
article :
doi10.1093/protein/gzt024
id25040
pubmed_id23754529
bacterial resistance :
Chloramphenicol
cloning :
backbonepCA24N
backbone_mutation
backbone_originKitagawa et al. (PMID:16769691)
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
For complete cloning information, please see the paper's supplement. For expression, E. coli DH5a-E cells harbouring each expression vector were grown at 37 C in LB broth containing chloramphenicol (34 g.ml-1), to OD600 ~ 0.6. IPTG (0.4 mM, final concentration) was added as an inducer, and protein over-expression was at 28 C for 16-18 h.
growth strain :
IPTG-inducible expression of ligase-pprA fusion for protein purification
origin :
37
pi :
alt_names
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoterT5-lac
sequencing_primer_3pCA24N.rev2 5'-CAAATCCAGATGGAGTTCTGAGG-3'
sequencing_primer_5pCA24N.for (GATAACAATTTCACACAGAATTCATTAAAGAG)
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesT4p202, lig
gene30
id1258680
genbank_ids
mutation
nameT4 DNA ligase
shRNA_sequence
size
species
32644
Other
tags
locationN terminal on backbone
tag6xHis
locationC terminal on insert
tagcodon optimized pprA (from Deinococcus radiodurans)
plasmid copy :
Previously, we PCR amplified the lig gene from pRBL (Ren et al., 1997; PMID: 9305776). The optimized pprA gene, with flanking restriction sites (BamHI and SpeI), was synthesized by DNA 2.0 (Menlo Park, CA, USA).
resistance markers :
3286
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA