pET151-Vps4(101-437)-Hcp1 | Addgene 87737 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pET151-Vps4(101-437)-Hcp1

catalog :

87737

citations: 3

Reference
Castanzo D, LaFrance B, Martin A. The AAA+ ATPase Msp1 is a processive protein translocase with robust unfoldase activity. Proc Natl Acad Sci U S A. 2020;117:14970-14977 pubmed publisher
Han H, Fulcher J, Dandey V, Iwasa J, Sundquist W, Kay M, et al. Structure of Vps4 with circular peptides and implications for translocation of two polypeptide chains by AAA+ ATPases. elife. 2019;8: pubmed publisher
Monroe N, Han H, Shen P, Sundquist W, Hill C. Structural basis of protein translocation by the Vps4-Vta1 AAA ATPase. elife. 2017;6: pubmed publisher

product information

Catalog Number :

87737

Product Name :

pET151-Vps4(101-437)-Hcp1

article :

doi	10.7554/eLife.24487
id	25515
pubmed_id	28379137

bacterial resistance :

Ampicillin

cloning :

backbone

pET151

backbone_mutation

backbone_origin

Invitrogen

backbone_size

5700

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Bacterial Expression

growth strain :

Bacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1

origin :

pi :

alt_names

Vps4

cloning

clone_method	Ligation Independent Cloning
cloning_site_3
cloning_site_5
promoter	T7
sequencing_primer_3	GCTAGTTATTGCTCAGCGG
sequencing_primer_5	TAATACGACTCACTATAGG
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	YPR173C, CSC1, DID6, END13, GRD13, VPL4, VPT10
gene	VPS4
id	856303

genbank_ids

NM_001184270

mutation

deleted amino acids 1-100

name

Vacuolar protein sorting-associated protein 4

shRNA_sequence

size

1554

species

4932

Saccharomyces cerevisiae