pcDNA-I-PhoCl-HCVp | Addgene 87697 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pcDNA-I-PhoCl-HCVp

catalog :

87697

citations: 2

Reference
Reed E, Schuster B, Good M, Hammer D. SPLIT: Stable Protein Coacervation Using a Light Induced Transition. ACS Synth Biol. 2020;9:500-507 pubmed publisher
Zhang W, Lohman A, Zhuravlova Y, Lu X, Wiens M, Hoi H, et al. Optogenetic control with a photocleavable protein, PhoCl. Nat Methods. 2017;14:391-394 pubmed publisher

product information

Catalog Number :

87697

Product Name :

pcDNA-I-PhoCl-HCVp

article :

doi	10.1038/nmeth.4222
id	25331
pubmed_id	28288123

bacterial resistance :

Ampicillin

cloning :

backbone

pcDNA3.1

backbone_mutation

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

Please note that there's a M to I discrepancy between the reference sequence and Addgene's quality control sequence. The change does not affect EBFP or PhoCI.

growth strain :

Express I-PhoCl-HCVp with N-term EBFP2 in mammalian cells

origin :

pi :

alt_names

cloning

clone_method	Unknown
cloning_site_3
cloning_site_5
promoter	CMV
sequencing_primer_3	BGH-rev
sequencing_primer_5	CMV-F
site_3_destroyed
site_5_destroyed

entrez_gene

genbank_ids

mutation

name

I-PhoCl-HCVp containing the Ki = 10.5 nM inhibitor (CP5-46A-4D5E)

shRNA_sequence

size

species

100

Synthetic