This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
RV-cag-Dio-Kif5c-2A-tdt
catalog :
87668
citations: 1
Reference
Lim L, Pakan J, Selten M, Marques Smith A, Llorca A, Bae S, et al. Optimization of interneuron function by direct coupling of cell migration and axonal targeting. Nat Neurosci. 2018;21:920-931 pubmed publisher
product information
Catalog Number :
87668
Product Name :
RV-cag-Dio-Kif5c-2A-tdt
article :
doi10.1038/s41593-018-0162-9
id25026
pubmed_id29915195
bacterial resistance :
Ampicillin
cloning :
backboneRV-Syn
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Retroviral
growth notes :
Please note that several discrepancies (including mutation N185S in EYFP, and mutations V40A, E125V in Kif5C) were found between Addgene's quality control and the depositor's sequence. The depositor noted that these discrepancies do NOT affect plasmid function. Kif5Cdelta560 subclone from addgene plasmid 45059, pBa.Kif5c 1-560.GFP
growth strain :
retroviral vector, conditional expression membrane Tdtomato and Kif5Cdelta560
origin :
37
pi :
alt_names
NM_001107730.1
cloning
clone_methodRestriction Enzyme
cloning_site_3AscI
cloning_site_5PacI
promoterCAG
sequencing_primer_3WPRE-R
sequencing_primer_5pRRL-Fwd
site_3_destroyed
site_5_destroyed
entrez_gene
aliases
geneKif5c
id311024
genbank_ids
mutationmotor domain aa1-560 present, V40A, E125V (see depositor comment below)
nameKif5C-P2A-mTdt
shRNA_sequence
size4002
species
10116
Rattus norvegicus
tags
locationN terminal on insert
tagpalmitylation
resistance markers :
3282
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA