This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pNCMV HPV8 E6 K136N
catalog :
87657
citations: 1
Reference
Meyers J, Uberoi A, Grace M, Lambert P, Munger K. Cutaneous HPV8 and MmuPV1 E6 Proteins Target the NOTCH and TGF-? Tumor Suppressors to Inhibit Differentiation and Sustain Keratinocyte Proliferation. PLoS Pathog. 2017;13:e1006171 pubmed publisher
product information
Catalog Number :
87657
Product Name :
pNCMV HPV8 E6 K136N
article :
doiPPATHOGENS-D-16-02140 [pii]
id25023
pubmed_id28107544
bacterial resistance :
Ampicillin
cloning :
backboneCMV Bam neo
backbone_mutationFlag/HA containing added at BamHI site
backbone_origin
backbone_size6550
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Flag/HA tagged HPV8 E6 K136N
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5BamHI
promoterCMV
sequencing_primer_3
sequencing_primer_5Bglobin intron F
site_3_destroyed1
site_5_destroyed
entrez_gene
genbank_ids
M12737.1
mutationK136N
nameHPV 8E6
shRNA_sequence
size542
species
32644
Other
HPV
tags
locationN terminal on backbone
tagFlag
locationN terminal on backbone
tagHA
plasmid copy :
The 8E6 template was given to us by Harold Pfister who originally cloned the HPV8 genome. (J. Virology 1986, Pfister H. et al.)
resistance markers :
351
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA