This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3-Venus-173-N-ORAI1
catalog :
87618
citations: 1
Reference
Hertel F, Mo G, Duwé S, Dedecker P, Zhang J. RefSOFI for Mapping Nanoscale Organization of Protein-Protein Interactions in Living Cells. Cell Rep. 2016;14:390-400 pubmed publisher
product information
Catalog Number :
87618
Product Name :
pcDNA3-Venus-173-N-ORAI1
article :
doi10.1016/j.celrep.2015.12.036
id25015
pubmed_id26748717
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3
backbone_mutation
backbone_originInvitrogen
backbone_size5500
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
N-terminal Venus (YFP) fragment fused to full-length ORAI1; for BiFC.
origin :
37
pi :
alt_names
ORAI1
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5BamHI
promoterCMV
sequencing_primer_3BGH reverse
sequencing_primer_5T7 forward
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCRACM1, IMD9, ORAT1, TAM2, TMEM142A
geneORAI1
id84876
genbank_ids
mutation
nameVenus173-N-ORAI1
shRNA_sequence
size1449
species
9606
Homo sapiens
100
Synthetic
tags
locationN terminal on backbone
tag6xHis
locationN terminal on backbone
tagT7 tag (gene 10 leader)
locationN terminal on backbone
tagXpress(TM) tag
plasmid copy :
ORAI1 cDNA provided by Dr. Paul Worley (Johns Hopkins)
resistance markers :
2182
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA