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product summary
company name :
Addgene
product type :
cDNA
product name :
2674 pBS Bcl-2 P1+P2
catalog :
8746
citations: 1
| Reference |
|---|
Young R, Korsmeyer S. A negative regulatory element in the bcl-2 5'-untranslated region inhibits expression from an upstream promoter. Mol Cell Biol. 1993;13:3686-97 pubmed
|
product information
Catalog Number :
8746
Product Name :
2674 pBS Bcl-2 P1+P2
article :
| doi | |
| id | 269 |
| pubmed_id | 8388542 |
bacterial resistance :
Ampicillin
cloning :
| backbone | pBluescript II KS(-) | |
| backbone_mutation | ||
| backbone_origin | Stratagene | |
| backbone_size | 3000 | |
| promoter | ||
| sequencing_primer_3 | ||
| sequencing_primer_5 | ||
| vector_types |
|
growth notes :
bcl-2 is from a genomic clone isolated from a normal human fibroblast library. To construct P1+P2Neo, a 279-bp polymerase chain reaction (PCR) product including the bcl-2 5'-untranslated region sequence from just 5' to the AccI site through base -1, relative to the initiation codon, (designated AccI/-1), was cloned into pBluescript KS(-) (Stratagene), using primergenerated 5' XhoI and 3' HindIII sites. Next, a 486-bp XhoI-AccI fragment from the 5'-untranslated region was cloned into this plasmid, producing a 756-bp XhoI/-1 (HindIII) clone. Addition of an approximately 2.2-kb XhoI fragment to this construct created a 3.0-kb bcl-2 P1+P2 clone. Finally, a 2.3-kb HindIII-BamHI fragment from RSVNeo (20) including the neomycin phosphotransferase (neo) gene and SV40 early splice and polyadenylation signals was inserted into the P1+P2 clone to produce P1+P2Neo.
origin :
37
pi :
|
resistance markers :
| 264 |
tags :
High Copy
terms :
| Neomycin (select with G418) |
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
headquarters: USA
questions and comments