FRB-CFP-ras | Addgene 87451 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

FRB-CFP-ras

catalog :

87451

citations: 1

Reference
Liu N, Yang Y, Ge L, Liu M, Colecraft H, Liu X. Cooperative and acute inhibition by multiple C-terminal motifs of L-type Ca2+ channels. elife. 2017;6: pubmed publisher

product information

Catalog Number :

87451

Product Name :

FRB-CFP-ras

article :

doi	10.7554/eLife.21989
id	25007
pubmed_id	28059704

bacterial resistance :

Ampicillin

cloning :

backbone

pcDNA3

backbone_mutation

backbone_origin

backbone_size

5388

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

ras fragment (60 bp)

growth strain :

rapamycin binding motif of mTOR (FRB) anchored to the plasma membrane

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	Xho I
cloning_site_5	Bam HI
promoter	CMV
sequencing_primer_3
sequencing_primer_5	CMV-F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	FRAP, FRAP1, FRAP2, RAFT1, RAPT1, SKS
gene	MTOR
id	2475

genbank_ids

mutation

FKBP and rapamycin binding domain only

name

FRB

shRNA_sequence

size

282

species

9606

Homo sapiens