This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pET42b-HF-BE3
catalog :
87438
citations: 2
Reference
Zou R, Liu Y, Wu B, Ha T. Cas9 deactivation with photocleavable guide RNAs. Mol Cell. 2021;81:1553-1565.e8 pubmed publisher
Rees H, Komor A, Yeh W, Caetano Lopes J, Warman M, Edge A, et al. Improving the DNA specificity and applicability of base editing through protein engineering and protein delivery. Nat Commun. 2017;8:15790 pubmed publisher
product information
Catalog Number :
87438
Product Name :
pET42b-HF-BE3
article :
doi10.1038/ncomms15790
id28189802
pubmed_id28585549
bacterial resistance :
Kanamycin
cloning :
backbonepET_42b
backbone_mutation
backbone_origin
backbone_size4983
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth strain :
Expresses HF-BE3-NLS with an N-terminal His Tag (His6) for bacterial expression
origin :
37
pi :
alt_names
GGS-His6-rAPOBEC1-XTEN-nHF-Cas9-GGS-UGI-GGS-NLS
cloning
clone_methodLigation Independent Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3GCTAGTTATTGCTCAGCGG
sequencing_primer_5TAATACGACTCACTATAGGG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesREPR, apobec-1
geneApobec1
id25383
genbank_ids
mutation
nameHF-BE3
shRNA_sequence
size5163
species
9606
Homo sapiens
10116
Rattus norvegicus
32644
Other
Bacillus phage PBS2
tags
locationN terminal on insert
tagHis6
resistance markers :
774
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA