AICSDP-8:TOMM20-mEGFP | Addgene 87423 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

AICSDP-8:TOMM20-mEGFP

catalog :

87423

citations: 4

Reference
Meurant S, Mauclet L, Dieu M, Arnould T, Eyckerman S, Renard P. Endogenous TOM20 Proximity Labeling: A Swiss-Knife for the Study of Mitochondrial Proteins in Human Cells. Int J Mol Sci. 2023;24: pubmed publisher
Hayes B, Zhu P, Wang M, Pfeifer C, Xia Y, Phan S, et al. Confinement plus myosin-II suppression maximizes heritable loss of chromosomes, as revealed by live-cell ChReporters. J Cell Sci. 2023;136: pubmed publisher
Wienert B, Nguyen D, Guenther A, Feng S, Locke M, Wyman S, et al. Timed inhibition of CDC7 increases CRISPR-Cas9 mediated templated repair. Nat Commun. 2020;11:2109 pubmed publisher
Roberts B, Haupt A, Tucker A, Grancharova T, Arakaki J, Fuqua M, et al. Systematic gene tagging using CRISPR/Cas9 in human stem cells to illuminate cell organization. Mol Biol Cell. 2017;28:2854-2874 pubmed publisher

product information

Catalog Number :

87423

Product Name :

AICSDP-8:TOMM20-mEGFP

article :

doi	10.1091/mbc.E17-03-0209
id	28192202
pubmed_id	28814507

bacterial resistance :

Kanamycin

cloning :

backbone

pUC57

backbone_mutation

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

CRISPR

Other

Donor Template

growth notes :

This plasmid has been used with locus-specific CRISPR/Cas9 to add a mEGFP tag to the C-terminus of human TOMM20 in WTC human induced pluripotent stem cells by the Allen Institute for Cell Science. Linker (AA) sequence: GGSGDPPVAT. After protein tagging using this donor template plasmid and CRISPR/Cas9 reagents, transfected cells may exhibit varying intensity levels of fluorescence, likely due to editing precision. To obtain cells of uniform intensity levels, see our protocol for fluorescence-assisted cell sorting and subcloning of transfected cells (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/ . Please visit https://www.biorxiv.org/content/early/2017/03/31/123042 for bioRxiv preprint.

growth strain :

Homology arms and linker-mEGFP sequence for C-terminus tagging of human TOMM20

origin :

pi :

alt_names

TOMM20

TOM20

cloning

clone_method	Unknown
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3	M13-Rev
sequencing_primer_5	M13-F20
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	MAS20, MOM19, TOM20
gene	TOMM20
id	9804

genbank_ids

mutation

homology arms contain point mutations to disrupt crRNA binding sites used and point mutations specific to WTC genome

name

TOMM20 Homology Arms with linker-mEGFP

shRNA_sequence

size

2747

species

9606

Homo sapiens