This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pHA-N1-GalT
catalog :
87326
citations: 1
Reference
Tie H, Mahajan D, Chen B, Cheng L, VanDongen A, Lu L. A novel imaging method for quantitative Golgi localization reveals differential intra-Golgi trafficking of secretory cargoes. Mol Biol Cell. 2016;27:848-61 pubmed publisher
product information
Catalog Number :
87326
Product Name :
pHA-N1-GalT
article :
doi10.1091/mbc.E15-09-0664
id17709
pubmed_id26764092
bacterial resistance :
Kanamycin
cloning :
backbonepHA-N1
backbone_mutationmCherry is replaced with HA, which is generated by annealed oligos flanking with BamHI and NotI.
backbone_originClontech
backbone_size4030
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
To express GalT-HA in mammalian cells, GalT encodes the 1-81 amino acids from 1,4-galactosyltransferase
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5NheI
promoterCMV-F
sequencing_primer_3
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesB4GAL-T1, CDG2D, GGTB2, GT1, GTB, beta4Gal-T1
geneB4GALT1
id2683
genbank_ids
NM_001497.3
mutation
name1-81 amino acids from 1,4-galactosyltransferase
shRNA_sequence
size243
species
9606
Homo sapiens
tags
locationC terminal on backbone
tagHA
resistance markers :
3183
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA