This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pAcGFP1-N1-SialT
catalog :
87324
citations: 1
Reference
Tie H, Mahajan D, Chen B, Cheng L, VanDongen A, Lu L. A novel imaging method for quantitative Golgi localization reveals differential intra-Golgi trafficking of secretory cargoes. Mol Biol Cell. 2016;27:848-61 pubmed publisher
product information
Catalog Number :
87324
Product Name :
pAcGFP1-N1-SialT
article :
doi10.1091/mbc.E15-09-0664
id17709
pubmed_id26764092
bacterial resistance :
Kanamycin
cloning :
backbonepAcGFP1-N1
backbone_mutation
backbone_originClontech
backbone_size4700
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
To express SialT-AcGFP1 in mammalian cells, SialT encodes the full length coding sequence of -galactosamide -2,6-sialyltransferase
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5XhoI
promoterCMV
sequencing_primer_3
sequencing_primer_5EGFP-N
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCDw75, SIAT1, ST6GalI, ST6N
geneST6GAL1
id6480
genbank_ids
NM_003032
mutation
nameST 6 beta-galactoside alpha-2,6-sialyltransferase 1
shRNA_sequence
size1221
species
9606
Homo sapiens
tags
locationC terminal on backbone
tagAcGFP1
resistance markers :
3183
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA