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product summary
company name :
Addgene
product type :
cDNA
product name :
pIZ-EGFP-MYO10-HMM
catalog :
87257
citations: 1
product information
Catalog Number :
87257
Product Name :
pIZ-EGFP-MYO10-HMM
article :
| doi | 10.1091/mbc.E16-08-0583 |
| id | 24971 |
| pubmed_id | 27932498 |
bacterial resistance :
Bleocin (Zeocin)
cloning :
| backbone | pIZ/V5-His | |
| backbone_mutation | When cloning into the MCS to generate a EGFP-Myo10 fusion protein, a stop codon must be included to prevent translation into the C-terminal V5/His tag. | |
| backbone_origin | Thermo Scientific | |
| backbone_size | 2900 | |
| promoter | ||
| sequencing_primer_3 | ||
| sequencing_primer_5 | ||
| vector_types |
|
growth strain :
Expression plasmid to perform NanoSPD assays in insect cells (generates EGFP-tagged bait).
growth temp :
Zeocin is sensitive to NaCl in standard LB and LB-Agar formulations. Use modified recipe, into 1L ddH20 add: 10g tryptone, 5g yeast extract, 5g NaCl.
origin :
37
pi :
|
resistance markers :
| 3272 |
tags :
High Copy
terms :
| Zeocin |
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
headquarters: USA
questions and comments