This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3.1-MYO10-HMM-Nanotrap
catalog :
87255
citations: 2
Reference
Bocanegra J, Fujita B, Melton N, Cowan J, Schinski E, Tamir T, et al. The MyMOMA domain of MYO19 encodes for distinct Miro-dependent and Miro-independent mechanisms of interaction with mitochondrial membranes. Cytoskeleton (Hoboken). 2019;: pubmed publisher
Bird J, Barzik M, Drummond M, Sutton D, Goodman S, Morozko E, et al. Harnessing molecular motors for nanoscale pulldown in live cells. Mol Biol Cell. 2017;28:463-475 pubmed publisher
product information
Catalog Number :
87255
Product Name :
pcDNA3.1-MYO10-HMM-Nanotrap
article :
doi10.1091/mbc.E16-08-0583
id24971
pubmed_id27932498
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.1(+)
backbone_mutation
backbone_originThermo Fisher
backbone_size5428
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Expression plasmid to perform NanoSPD 2.0 assays in mammalian cells.
origin :
37
pi :
alt_names
Unconventional myosin 10
cloning
clone_methodLigation Independent Cloning
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3BGH_rev_primer
sequencing_primer_5CMV_fwd_primer
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAW048724, D15Ertd600, D15Ertd600e, mKIAA0799, myos, myosin-X
geneMyo10
id17909
genbank_ids
NM_019472.2
mutationMyo10 is truncated to contain aa.1-941
nameMYO10
shRNA_sequence
size2823
species
10090
Mus musculus
tags
locationC terminal on insert
tagGFP-nanotrap
resistance markers :
3272
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA