This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
hisSUMO-mZFP57
catalog :
87224
citations: 1
Reference
Zuo Z, Roy B, Chang Y, Granas D, Stormo G. Measuring quantitative effects of methylation on transcription factor-DNA binding affinity. Sci Adv. 2017;3:eaao1799 pubmed publisher
product information
Catalog Number :
87224
Product Name :
hisSUMO-mZFP57
article :
doiaao1799 [pii]
id28192359
pubmed_id29159284
bacterial resistance :
Ampicillin
cloning :
backboneNEB DHFR control plasmid
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
This construct has the DNA binding domain of mouse ZFP57 including two separate groups of zinc fingers and the linker in between. In our published work about methylation sensitivity assay, only the truncated first group of Zinc finger (F1-F3) was used for Methyl-Spec-seq assay.
growth strain :
Express hisSUMO tagged Zinc finger protein 57 F1-F3(mouse) in E. coli
origin :
37
pi :
alt_names
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterT7
sequencing_primer_3T7 terminator
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesG19, Zfp-57
geneZfp57
id22715
genbank_ids
mutation
nameZFP57
shRNA_sequence
size
species
10090
Mus musculus
tags
locationN terminal on backbone
taghisSUMO
resistance markers :
3632
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA