pEFIRES-P-EGFP-ADRP | Addgene 87157 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pEFIRES-P-EGFP-ADRP

catalog :

87157

citations: 1

Reference
Salo V, Belevich I, Li S, Karhinen L, Vihinen H, Vigouroux C, et al. Seipin regulates ER-lipid droplet contacts and cargo delivery. EMBO J. 2016;35:2699-2716 pubmed

product information

Catalog Number :

87157

Product Name :

pEFIRES-P-EGFP-ADRP

article :

doi	10.15252/embj.201695170
id	23093
pubmed_id	27879284

bacterial resistance :

Ampicillin

cloning :

backbone

pEFIRES-P

backbone_mutation

backbone_origin

backbone_size

5689

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

This vector was generated by Simon Pfisterer and Shiqian Li (Elina Ikonen Lab). Benefits of using pEFIRES-P vector for stable overexpression in mammalian cells were described by Stephen Hobbs et al. (Biochem Biophys Res Commun. 1998 Nov 18;252(2):368-72.). pEFIRES-P was a gift from Dr. Olli Ritvos (University of Helsinki).

growth strain :

Fluorescent protein localized to lipid droplets (LDs)

origin :

pi :

alt_names

PLIN2

cloning

clone_method	Restriction Enzyme
cloning_site_3	XhoI
cloning_site_5	XhoI
promoter	mCherry-R TTGGTCACCTTCAGCTTGG
sequencing_primer_3	pEF-rev acaccggccttattccaagc
sequencing_primer_5	EGFP-C-F catggtcctgctggagttcgtg
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	ADFP, ADRP
gene	PLIN2
id	123

genbank_ids

BC005127.2

mutation

name

ADRP

shRNA_sequence

size

1314

species

9606

Homo sapiens