This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCMV4-ApoE4
catalog :
87087
citations: 2
Reference
Wu Y, Zhao F, Sure V, Ibrahim A, Yu C, Carr S, et al. Human ApoE2 Endows Stronger Contractility in Rat Cardiomyocytes Enhancing Heart Function. Cells. 2023;12: pubmed publisher
Hudry E, Dashkoff J, Roe A, Takeda S, Koffie R, Hashimoto T, et al. Gene transfer of human Apoe isoforms results in differential modulation of amyloid deposition and neurotoxicity in mouse brain. Sci Transl Med. 2013;5:212ra161 pubmed publisher
product information
Catalog Number :
87087
Product Name :
pCMV4-ApoE4
article :
doi10.1126/scitranslmed.3007000
id23074
pubmed_id24259049
bacterial resistance :
Ampicillin
cloning :
backbonepCMV4
backbone_mutation
backbone_origin
backbone_size5039
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
the plasmid used in the reference is in AAV backbone. the plasmid deposit here is not in AAV backbone
growth strain :
Expresses ApoE4 in Mammalian cells
growth temp :
Try following protocols for low copy plasmids if bacterial culture grow slowly.
origin :
37
pi :
alt_names
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3GGCACTGGAGTGGCAACTTC
sequencing_primer_5CCATTGACGTCAATGGGAGTTTG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAD2, APO-E, ApoE4, LDLCQ5, LPG
geneAPOE
id348
genbank_ids
NG_007084.2
mutation
nameApolipoprotein E4
shRNA_sequence
size953
species
9606
Homo sapiens
tags
resistance markers :
3257
tags :
Unknown
terms :
Other
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA