This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
6xHis-TEV-GEI-17(133-509)
catalog :
87056
citations: 1
Reference
Pelisch F, Sonneville R, Pourkarimi E, Agostinho A, Blow J, Gartner A, et al. Dynamic SUMO modification regulates mitotic chromosome assembly and cell cycle progression in Caenorhabditis elegans. Nat Commun. 2014;5:5485 pubmed publisher
product information
Catalog Number :
87056
Product Name :
6xHis-TEV-GEI-17(133-509)
article :
doi10.1038/ncomms6485
id23067
pubmed_id25475837
bacterial resistance :
Kanamycin
cloning :
backbonepHISTEV30a
backbone_mutation
backbone_origin
backbone_size5365
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
This plasmid has a GAGGGS linker that improves TEV cleavage efficiency.
growth strain :
Expresses 6xHis-tagged gei-17 (isoform f), with a TEV protease cleavage site between the tag and the ORF
origin :
37
pi :
alt_names
C. elegans SUMO E3 ligase (PIAS family)
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterT7
sequencing_primer_3T7term
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCELE_W10D5.3
genegei-17
id172733
genbank_ids
mutation
nameGEI-17 isfoomr f (aa 133-509)
shRNA_sequence
size
species
6239
Caenorhabditis elegans
tags
locationN terminal on backbone
tag6xHis-TEV
locationN terminal on insert
tagGAGGGS linker
resistance markers :
3258
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA