This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA5/FRT/TO-FLAG-CHK1
catalog :
86875
citations: 3
Reference
Li J, Zhao H, McMahon A, Yan S. APE1 assembles biomolecular condensates to promote the ATR-Chk1 DNA damage response in nucleolus. Nucleic Acids Res. 2022;50:10503-10525 pubmed publisher
Hiregange D, Naick H, Rao B. ATR signalling mediates the prosurvival function of phospho-NPM against PIDDosome mediated cell death. Cell Signal. 2020;71:109602 pubmed publisher
Xu Y, Anderson D, Ye Y. The HECT domain ubiquitin ligase HUWE1 targets unassembled soluble proteins for degradation. Cell Discov. 2016;2:16040 pubmed
product information
Catalog Number :
86875
Product Name :
pcDNA5/FRT/TO-FLAG-CHK1
article :
doi10.1038/celldisc.2016.40
id23015
pubmed_id27867533
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA 5/FR/TO
backbone_mutation
backbone_origin
backbone_size5070
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
To express CHK1 with a FLAG tag at N-terminus.
origin :
37
pi :
alt_names
CHK1
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5AflII
promoterCMV
sequencing_primer_3BGH-R
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCHK1, OZEMA21
geneCHEK1
id1111
genbank_ids
NM_001274.5.
mutationnone
nameCHEK1
shRNA_sequence
size1431
species
9606
Homo sapiens
tags
locationN terminal on insert
tagFLAG
resistance markers :
835
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA