This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA5/FRT/TO-GRB2-GFP
catalog :
86873
citations: 1
Reference
Xu Y, Anderson D, Ye Y. The HECT domain ubiquitin ligase HUWE1 targets unassembled soluble proteins for degradation. Cell Discov. 2016;2:16040 pubmed
product information
Catalog Number :
86873
Product Name :
pcDNA5/FRT/TO-GRB2-GFP
article :
doi10.1038/celldisc.2016.40
id23015
pubmed_id27867533
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA 5/FR/TO
backbone_mutation
backbone_origin
backbone_size5070
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
To express GRB2 with a GFP tag at C terminus.
origin :
37
pi :
alt_names
SH2/SH3 adapter GRB2
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5BamHI
promoterCMV
sequencing_primer_3BGH-R
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesASH, EGFRBP-GRB2, Grb3-3, MST084, MSTP084, NCKAP2
geneGRB2
id2885
genbank_ids
NM_002086.4
mutationnone
nameGRB2
shRNA_sequence
size651
species
9606
Homo sapiens
tags
locationC terminal on insert
tagGFP
resistance markers :
835
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA