pEGFP-C1-AR V7 | Addgene 86856 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pEGFP-C1-AR V7

catalog :

86856

citations: 3

Reference
Kelava I, Chiaradia I, Pellegrini L, Kalinka A, Lancaster M. Androgens increase excitatory neurogenic potential in human brain organoids. Nature. 2022;602:112-116 pubmed publisher
Yang Z, Wang D, Johnson J, Pascal L, Takubo K, Avula R, et al. A Novel Small Molecule Targets Androgen Receptor and Its Splice Variants in Castration-Resistant Prostate Cancer. Mol Cancer Ther. 2020;19:75-88 pubmed publisher
Szafran A, Stephan C, Bolt M, Mancini M, Marcelli M, Mancini M. High-Content Screening Identifies Src Family Kinases as Potential Regulators of AR-V7 Expression and Androgen-Independent Cell Growth. Prostate. 2017;77:82-93 pubmed publisher

product information

Catalog Number :

86856

Product Name :

pEGFP-C1-AR V7

article :

doi	10.1002/pros.23251
id	23014
pubmed_id	27699828

bacterial resistance :

Kanamycin

cloning :

backbone

pEGFP-C1

backbone_mutation

none

backbone_origin

Clontech (Takara)

backbone_size

4731

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

The poly-glutamine repeat region in this plasmid is known to be unstable and the exact number of repeats may vary. However, repeats of 18-26 glutamines are all considered wildtype. Screen multiple colonies to ensure isolation of a plasmid with a suitable number of repeats. Prostate. 2013 February 15; 73(3): 267 277. doi:10.1002/pros.22566. The activity of the androgen receptor variant AR-V7 is regulated by FOXO1 in a PTEN-PI3K-AKT-dependent way. Cloning is described in the supplemental materials (excerpted below). Cloning Strategy The carboxy-terminal part of AR-V7 and AR-V1 were PCR amplified by using the following primers: sense (common for AR-V1 and AR-V7 amplification) 5 -GCGCAAGCTTCTGGGTGTCACTATGGAGC (harboring a Hind III site), antisense for AR-V7 GCTCTAGATCAGGGTCTGGTCATTTTGAGATGC (harboring a XbaI site), antisense for AR-V1 GCTCTAGATTAAGGAAGCCATTCTGAGACTCC (also harboring a XbaI site). CMV-AR-V7 and CMV-AR-V1 were generated by replacing a HindIII-XbaI cDNA fragment encoding the carboxy-terminal portion of wild-type AR (subcloned into a pcDNA3 plasmid) with the AR-V7 or AR-V1 fragments generated by PCR. GFP-AR-V7 and GFP-AR-V1 were generated by replacing an XmaI-XbaI cDNA fragment encoding the carboxy-terminal part of wild-type AR, subcloned in frame to the green fluorescent protein of plasmid pEGFP-c1, with XmaI-XbaI fragments from the pcDNA3 plasmids containing the AR-V7 and AR-V1 variants.

growth strain :

Fluorescent human androgen-receptor splice variant 7, lacking the ligand-binding domain (fused to EGFP)

growth temp :

Sequence-verify the poly-glutamine expansion when preparing new plasmid preps. Depositor recommends screening a handful of colonies. See Depositor Comments for more information.

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	Xba I
cloning_site_5	Xma I
promoter	CMV
sequencing_primer_3
sequencing_primer_5	EGFP-C
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	AIS, AR8, DHTR, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM
gene	AR
id	367

genbank_ids

NM_000044.3

mutation

Alternative splice variant 7 (alteration/deletion of the C-terminus)

name

Androgen receptor (AR)

shRNA_sequence

size

1932

species

9606

Homo sapiens