This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
MSCV-(mcs) human CSF1 full length-IRES-Puro
catalog :
86797
citations: 2
Reference
Samain R, Brunel A, Douche T, Fanjul M, Cassant Sourdy S, Rochotte J, et al. Pharmacological Normalization of Pancreatic Cancer-Associated Fibroblast Secretome Impairs Pro-metastatic Cross-Talk With Macrophages. Cell Mol Gastroenterol Hepatol. 2021;: pubmed publisher
Roussel M, Dull T, Rettenmier C, Ralph P, Ullrich A, Sherr C. Transforming potential of the c-fms proto-oncogene (CSF-1 receptor). Nature. 1987;325:549-52 pubmed
product information
Catalog Number :
86797
Product Name :
MSCV-(mcs) human CSF1 full length-IRES-Puro
article :
doi10.1038/325549a0
id22994
pubmed_id3027579
bacterial resistance :
Ampicillin
cloning :
backboneMSCV-IRES-Puro
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
growth notes :
Full length CSF-1 is secreted
growth strain :
retroviral expression of CSF-1
origin :
37
pi :
alt_names
macrophage colony stimulating factor 1
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5XhoI
promoter
sequencing_primer_3pCDH-rev
sequencing_primer_5pLXSN5; pBABE5
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCSF-1, MCSF, PG-M-CSF
geneCSF1
id1435
genbank_ids
mutation
nameCSF-1
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationC terminal on backbone
tagIRES-Puro
plasmid copy :
The mcs is from the pBabe vector that was inserted into the MSCV vector to provide more cloning sites, and was developed by Dr Den Besten. The human CSF-1 cDNAs were cloned by the Cetus Company.
resistance markers :
1113
1112
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA