This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pRB2963
catalog :
8678
citations: 1
| Reference |
|---|
Anders K, Botstein D. Dominant-lethal alpha-tubulin mutants defective in microtubule depolymerization in yeast. Mol Biol Cell. 2001;12:3973-86 pubmed
|
product information
Catalog Number :
8678
Product Name :
pRB2963
article :
| doi | 10.1091/mbc.12.12.3973 |
| id | 255 |
| pubmed_id | 11739794 |
bacterial resistance :
Ampicillin
cloning :
| backbone | pTS408 | |
| backbone_mutation | ||
| backbone_origin | ||
| backbone_size | ||
| promoter | ||
| sequencing_primer_3 | ||
| sequencing_primer_5 | ||
| vector_types |
|
growth notes :
PCR amplified TUB1-828 DNA were digested with BamHI and SpeI and ligated to the BamHI and XbaI sites of pTS408. pTS408 was constructed by PCR amplifying GFP with BamHI and BglII sites and by inserting this into the BamHI site of pTS210, creating a plasmid with the polylinker downstream of GFP to create NH2-terminal GFP fusions.The vector plasmid pTS210, a YCp50-based construct, contained the GAL1/GAL10 promoter, a short polylinker (BamHI, HindIII, XbaI), and the ACT1 transcriptional terminator.
growth temp :
Don't know
inserts :
Unknown
origin :
37
pi :
|
resistance markers :
| 48 |
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
headquarters: USA
questions and comments