This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pRB2958
catalog :
8676
citations: 1
| Reference |
|---|
Anders K, Botstein D. Dominant-lethal alpha-tubulin mutants defective in microtubule depolymerization in yeast. Mol Biol Cell. 2001;12:3973-86 pubmed
|
product information
Catalog Number :
8676
Product Name :
pRB2958
article :
| doi | 10.1091/mbc.12.12.3973 |
| id | 255 |
| pubmed_id | 11739794 |
bacterial resistance :
Ampicillin
cloning :
| backbone | pTS210 | |
| backbone_mutation | ||
| backbone_origin | ||
| backbone_size | ||
| promoter | ||
| sequencing_primer_3 | ||
| sequencing_primer_5 | ||
| vector_types |
|
growth notes :
tub1-827 was from Richards et al 2000 Mol. Biol. Cell 11, 1887-1903. It was PCRed, and the PCR products were digested with BamHI and SpeI and ligated to the BamHI and XbaI sites of pTS210. The vector plasmid pTS210, a YCp50-based construct, contained the GAL1/GAL10 promoter, a short polylinker (BamHI, HindIII, XbaI), and the ACT1 transcriptional terminator.
growth temp :
Don't know
inserts :
Unknown
origin :
37
pi :
|
resistance markers :
| 48 |
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
headquarters: USA
questions and comments