pRB2956 | Addgene 8675 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pRB2956

catalog :

8675

citations: 1

Reference
Anders K, Botstein D. Dominant-lethal alpha-tubulin mutants defective in microtubule depolymerization in yeast. Mol Biol Cell. 2001;12:3973-86 pubmed

product information

Catalog Number :

8675

Product Name :

pRB2956

article :

doi	10.1091/mbc.12.12.3973
id	255
pubmed_id	11739794

bacterial resistance :

Ampicillin

cloning :

backbone

pTS210

backbone_mutation

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Yeast Expression

growth notes :

PCRed TUB1-820 (from Richards et al 2000 Mol. Biol. Cell 11, 1887-1903) products were digested with BamHI and SpeI and ligated to the BamHI and XbaI sites of pTS210. The vector plasmid pTS210, a YCp50-based construct, contained the GAL1/GAL10 promoter, a short polylinker (BamHI, HindIII, XbaI), and the ACT1 transcriptional terminator.

growth temp :

Don't know

inserts :

Unknown

origin :

pi :

alt_names

TUB1-820

TUB1

cloning

clone_method	Restriction Enzyme
cloning_site_3	See comment
cloning_site_5	See comment
promoter
sequencing_primer_3
sequencing_primer_5	N/A
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	YML085C
gene	TUB1
id	854889

genbank_ids

mutation

name

TUB1-820 under GAL1 promoter

shRNA_sequence

size

species

4932

Saccharomyces cerevisiae