This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pRB2067
catalog :
8670
citations: 1
| Reference |
|---|
Richards K, Anders K, Nogales E, Schwartz K, Downing K, Botstein D. Structure-function relationships in yeast tubulins. Mol Biol Cell. 2000;11:1887-903 pubmed
|
product information
Catalog Number :
8670
Product Name :
pRB2067
article :
| doi | 10.1091/mbc.11.5.1887 |
| id | 254 |
| pubmed_id | 10793159 |
bacterial resistance :
Ampicillin
cloning :
| backbone | pUC119 | |
| backbone_mutation | ||
| backbone_origin | ||
| backbone_size | ||
| promoter | ||
| sequencing_primer_3 | ||
| sequencing_primer_5 | ||
| vector_types |
|
growth notes :
UB1 fragment (SphI-SacI) from pRB306 inserted into SphI-SacI-cut polylinker of pUC119. Then, SalI-KpnI (partial) fragment from pJJ217 containing the entire HIS3 gene and flanking regions, inserted into XhoI-KpnI vector fragment; HIS3 is in the same orientation that TUB1 was; note that this disruption construct removes the first 763 bp of the 1580-bp ORF downstream of TUB1 (YML086c). For information on pUC119, search for pUC119 in Addgene's vector db.
growth temp :
Don't know
inserts :
Unknown
origin :
37
pi :
|
resistance markers :
| 48 |
tags :
Unknown
terms :
| HIS3 |
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
headquarters: USA
questions and comments