This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pRB2065
catalog :
8669
citations: 1
Reference
Richards K, Anders K, Nogales E, Schwartz K, Downing K, Botstein D. Structure-function relationships in yeast tubulins. Mol Biol Cell. 2000;11:1887-903 pubmed
product information
Catalog Number :
8669
Product Name :
pRB2065
article :
doi10.1091/mbc.11.5.1887
id254
pubmed_id10793159
bacterial resistance :
Ampicillin
cloning :
backbonepUC119
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Yeast Expression
growth notes :
TUB1 fragment (SphI-SacI) from pRB306 inserted into SphI-SacI-cut polylinker of pUC119. Then, BamHI-SalI fragment from pJJ282 (LEU2 gene) Klenow filled and inserted into the HpaI site, downstream of TUB1; LEU2 is oriented in the same direction as TUB1. For information on pUC119, search for pUC119 in Addgene's vector db.
growth temp :
Don't know
inserts :
Unknown
origin :
37
pi :
alt_names
TUB1
cloning
clone_methodRestriction Enzyme
cloning_site_3See comments
cloning_site_5See comments
promoter
sequencing_primer_3
sequencing_primer_5N/A
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesYML085C
geneTUB1
id854889
genbank_ids
mutation
nameTUB1 upstream of LEU2
shRNA_sequence
size
species
4932
Saccharomyces cerevisiae
tags
resistance markers :
48
tags :
Unknown
terms :
LEU2
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA