This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pHAGE2 mCherry-Rtn4a
catalog :
86683
citations: 2
Reference
Gao G, Zhu C, Liu E, Nabi I. Reticulon and CLIMP-63 regulate nanodomain organization of peripheral ER tubules. PLoS Biol. 2019;17:e3000355 pubmed publisher
Wang S, Tukachinsky H, Romano F, Rapoport T. Cooperation of the ER-shaping proteins atlastin, lunapark, and reticulons to generate a tubular membrane network. elife. 2016;5: pubmed publisher
product information
Catalog Number :
86683
Product Name :
pHAGE2 mCherry-Rtn4a
article :
doi10.7554/eLife.18605
id22962
pubmed_id27619977
bacterial resistance :
Ampicillin
cloning :
backbonepHAGE2 mCherry N-terminal
backbone_mutation
backbone_origin
backbone_size8200
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth notes :
Addgene NGS results found P122Q and P145T within the Rtn4a translation compared to NP_065393.1.
growth strain :
Lentivirus to stably express fluorescent human protein in mammalian cells
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5XhoI
promoterCMV
sequencing_primer_3CATATAGACAAACGCACACC
sequencing_primer_5GGTCTATATAAGCAGAGCTCG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesASY, NI220/250, NOGO, NSP, NSP-CL, Nbla00271, Nbla10545, RTN-X, RTN4-A, RTN4-B1, RTN4-B2, RTN4-C
geneRTN4
id57142
genbank_ids
mutation
nameRtn4a
shRNA_sequence
size3576
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagmCherry
plasmid copy :
Yoko Shibata
resistance markers :
411
tags :
High Copy
terms :
Blasticidin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA