This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pRB327
catalog :
8668
citations: 1
Reference
Schatz P, Solomon F, Botstein D. Genetically essential and nonessential alpha-tubulin genes specify functionally interchangeable proteins. Mol Cell Biol. 1986;6:3722-33 pubmed
product information
Catalog Number :
8668
Product Name :
pRB327
article :
doi
id253
pubmed_id3540600
bacterial resistance :
Ampicillin
cloning :
backboneYEp21
backbone_mutation
backbone_originBotstein et al. Gene. 1979 8(1):17-24.
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Yeast Expression
growth notes :
A TUB1 fragment was ligated from the SphI site (1.1 kilobases before the start codon) to a BglII site (0.5 kb beyond the stop codon) in place of the small SphI-to-BamHI (??) fragment of YEp21. For more information on YEp21, search for YEp21 in Addgene's vector db.
growth temp :
Don't know
inserts :
Unknown
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3See comments
cloning_site_5See comments
promoter
sequencing_primer_3
sequencing_primer_5N/A
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesYML085C
geneTUB1
id854889
genbank_ids
mutation
nameTUB1
shRNA_sequence
size
species
4932
Saccharomyces cerevisiae
tags
resistance markers :
48
tags :
Unknown
terms :
LEU2
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA