This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
ATP1A1_plasmid_donor_RD
catalog :
86551
citations: 1
Reference
Agudelo D, Duringer A, Bozoyan L, Huard C, Carter S, Loehr J, et al. Marker-free coselection for CRISPR-driven genome editing in human cells. Nat Methods. 2017;14:615-620 pubmed publisher
product information
Catalog Number :
86551
Product Name :
ATP1A1_plasmid_donor_RD
article :
doi10.1038/nmeth.4265
id25576
pubmed_id28417998
bacterial resistance :
Ampicillin
cloning :
backbonepUC19
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
CRISPR
TALEN
Other
Co-selection via HDR using ouabain
growth strain :
Homology-directed repair (HDR) donor plasmid with homology arms to ATP1A1 to introduce the Q118R and N129D (RD) mutations conferring cellular resistance to ouabain
origin :
37
pi :
alt_names
ATPase Na+/K+ transporting subunit alpha 1
Gene ID: 476
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5Acc65I
promoter
sequencing_primer_3tgagcgaggaagcggaagag
sequencing_primer_5cagggttattgtctcatgagcgg
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCMT2DD, HOMGSMR2
geneATP1A1
id476
genbank_ids
476
mutationQ118R, N129D
nameATP1A1
shRNA_sequence
size
species
9606
Homo sapiens
tags
resistance markers :
2506
tags :
High Copy
terms :
Other
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA