p4028 GST-E6AP | Addgene 8655 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

p4028 GST-E6AP

catalog :

8655

citations: 2

Reference
Beasley S, Kellum C, Orlomoski R, Idrizi F, Spratt D. An Angelman syndrome substitution in the HECT E3 ubiquitin ligase C-terminal Lobe of E6AP affects protein stability and activity. PLoS ONE. 2020;15:e0235925 pubmed publisher
Oda H, Kumar S, Howley P. Regulation of the Src family tyrosine kinase Blk through E6AP-mediated ubiquitination. Proc Natl Acad Sci U S A. 1999;96:9557-62 pubmed

product information

Catalog Number :

8655

Product Name :

p4028 GST-E6AP

article :

doi	10.1073/pnas.96.17.9557
id	249
pubmed_id	10449731

bacterial resistance :

Ampicillin

cloning :

backbone

pGEX-2T

backbone_mutation

backbone_origin

backbone_size

4948

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Bacterial Expression

growth notes :

See author's map. E6-AP is missing the first 22 amino acids of isoform 1 (protein starts with MEACT NEFCA). There is also an N->K mutation 20 amino acids downstream from the beginning of the insert. This mutation has no known functional ramifications.

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	EcoRI
cloning_site_5	BamHI
promoter
sequencing_primer_3
sequencing_primer_5	pGEX-5'
site_3_destroyed	1
site_5_destroyed

entrez_gene

aliases	ANCR, AS, E6-AP, EPVE6AP, HPVE6A, PIX1
gene	UBE3A
id	7337

genbank_ids

mutation

name

E6-AP

shRNA_sequence

size

2500

species

9606

Homo sapiens