This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
CRAF_S29A_pcDNA3.1
catalog :
86506
citations: 1
Reference
Rose J, Huang P, Camp N, Ye J, Leidal A, Goreshnik I, et al. A computationally engineered RAS rheostat reveals RAS-ERK signaling dynamics. Nat Chem Biol. 2017;13:119-126 pubmed publisher
product information
Catalog Number :
86506
Product Name :
CRAF_S29A_pcDNA3.1
article :
doi10.1038/nchembio.2244
id22808
pubmed_id27870838
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.1
backbone_mutation
backbone_origin
backbone_size5574
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Expresses CRAF_S29A in mammalian cells [Edit]
origin :
37
pi :
alt_names
RAF1
Raf-1 proto-oncogene, serine/threonine kinase
C-RAF
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3BGHR
sequencing_primer_5CMV_F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCMD1NN, CRAF, NS5, Raf-1, c-Raf
geneRAF1
id5894
genbank_ids
NM_002880.3
mutationS29A
nameCRAF
shRNA_sequence
size1977
species
9606
Homo sapiens
tags
locationN terminal on insert
tagFLAG
resistance markers :
943
tags :
High Copy
terms :
Hygromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA