This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
xCIAR_pcDNA5/FRT/TO
catalog :
86504
citations: 1
Reference
Rose J, Huang P, Camp N, Ye J, Leidal A, Goreshnik I, et al. A computationally engineered RAS rheostat reveals RAS-ERK signaling dynamics. Nat Chem Biol. 2017;13:119-126 pubmed publisher
product information
Catalog Number :
86504
Product Name :
xCIAR_pcDNA5/FRT/TO
article :
doi10.1038/nchembio.2244
id22808
pubmed_id27870838
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA5/FRT/TO
backbone_mutation
backbone_originInvitrogen
backbone_size5137
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Other
FRT
growth strain :
Expresses inactive control CIAR (xCIAR) in mammalian cells. Used to generate Flp-In stables.
origin :
37
pi :
alt_names
xS.17.3
inactive control Chemically Inducible Activator of RAS
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterCMV (tet operator)
sequencing_primer_3BGHR
sequencing_primer_5CMV_F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesGF1, GGF1, GINGF, HGF, NS4, SOS-1
geneSOS1
id6654
genbank_ids
mutationF929A and T968L in SOScat
namexCIAR
shRNA_sequence
size2202
species
9606
Homo sapiens
tags
resistance markers :
943
tags :
High Copy
terms :
Hygromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA