pEGFP-C1-AR Q48 | Addgene 86428 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pEGFP-C1-AR Q48

catalog :

86428

citations: 1

Reference
Stenoien D, Cummings C, Adams H, Mancini M, Patel K, DeMartino G, et al. Polyglutamine-expanded androgen receptors form aggregates that sequester heat shock proteins, proteasome components and SRC-1, and are suppressed by the HDJ-2 chaperone. Hum Mol Genet. 1999;8:731-41 pubmed

product information

Catalog Number :

86428

Product Name :

pEGFP-C1-AR Q48

article :

doi	10.1093/hmg/8.5.731
id	4075
pubmed_id	10196362

bacterial resistance :

Kanamycin

cloning :

backbone

pEGFP-C1

backbone_mutation

none

backbone_origin

Clontech (Takara)

backbone_size

4731

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

Addgene's sequencing results found that this plasmid carries an extra Q in the poly-glutamine expansion bring the total to 49 rather than 48 and found a G130D mutation compared to NCBI reference sequences. These mutations are not known to affect plasmid function.

growth strain :

Fluorescent human androgen receptor (fused to EGFP) with expanded polyglutamine region

growth temp :

New plasmid preps must be sequenced to verify (there can be loss or addition of poly-glutamine repeats).

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	BglII
cloning_site_5	Xma I
promoter	CMV
sequencing_primer_3
sequencing_primer_5	EGFP-C
site_3_destroyed	1
site_5_destroyed

entrez_gene

aliases	AIS, AR8, DHTR, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM
gene	AR
id	367

genbank_ids

NM_000044.4

mutation

Poly-glutamine expansion, G130D

name

Androgen receptor (AR)

shRNA_sequence

size

2850

species

9606

Homo sapiens