This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
PX458_MIXL1_1
catalog :
86335
citations: 1
Reference
Savic D, Partridge E, Newberry K, Smith S, Meadows S, Roberts B, et al. CETCh-seq: CRISPR epitope tagging ChIP-seq of DNA-binding proteins. Genome Res. 2015;25:1581-9 pubmed publisher
product information
Catalog Number :
86335
Product Name :
PX458_MIXL1_1
article :
doi10.1101/gr.193540.115
id15802
pubmed_id26355004
bacterial resistance :
Ampicillin
cloning :
backbonePX458
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
CRISPR
growth notes :
Note: This plasmid is part of the Mendenhall and Meyers CRISPR-based Tagging system to add a tag (currently using FLAG) to endogenous proteins. Please see https://www.addgene.org/crispr/tagging/ for more details.
growth strain :
Encodes gRNA for 3' target of human MIXL1
origin :
37
pi :
alt_names
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5pBR322ori-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesMILD1, MIX, MIXL
geneMIXL1
id83881
genbank_ids
mutation
namegRNA against MIXL1
shRNA_sequenceGAGGATTCTGGGAGAATTC
size
species
9606
Homo sapiens
tags
resistance markers :
1728
2438
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA