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product summary
company name :
Addgene
product type :
cDNA
product name :
pFETCh_ZGPAT
catalog :
86276
citations: 1
product information
Catalog Number :
86276
Product Name :
pFETCh_ZGPAT
article :
| doi | 10.1101/gr.193540.115 |
| id | 15802 |
| pubmed_id | 26355004 |
bacterial resistance :
Kanamycin
cloning :
| backbone | pFETCh_Donor | |
| backbone_mutation | ||
| backbone_origin | ||
| backbone_size | ||
| promoter | ||
| sequencing_primer_3 | ||
| sequencing_primer_5 | ||
| vector_types |
|
growth notes :
This plasmid was co-transfected along with PX458_ZGPAT_1 and PX458_ZGPAT_2 into HepG2 cells, and genomic editing was verified by PCR. We performed ChIP-seq on this cell line using anti-FLAG Sigma F1804 and obtained data that passed our quality tests. Immunoprecipitation with anti-FLAG Sigma F1804 followed by mass spectrometry identified ZGPAT. All released data, including ChIP-seq files and validation data, are available at the ENCODE web portal: https://www.encodeproject.org/search/?searchTerm=flag&type=Experiment&lab.title=Richard+Myers%2C+HAIB . Note: This plasmid is part of the Mendenhall and Meyers CRISPR-based Tagging system to add a tag (currently using FLAG) to endogenous proteins. Please see https://www.addgene.org/crispr/tagging/ for more details.
growth strain :
Donor vector for 3' FLAG tag of human ZGPAT
origin :
37
pi :
|
resistance markers :
| 1728 |
| 2438 |
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
headquarters: USA
questions and comments