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product summary
company name :
Addgene
product type :
cDNA
product name :
pFETCh_MIER3
catalog :
86274
citations: 1
product information
Catalog Number :
86274
Product Name :
pFETCh_MIER3
article :
| doi | 10.1101/gr.193540.115 |
| id | 15802 |
| pubmed_id | 26355004 |
bacterial resistance :
Kanamycin
cloning :
| backbone | pFETCh_Donor | |
| backbone_mutation | ||
| backbone_origin | ||
| backbone_size | ||
| promoter | ||
| sequencing_primer_3 | ||
| sequencing_primer_5 | ||
| vector_types |
|
growth notes :
This plasmid was co-transfected along with PX458_MIER3_1 and PX458_MIER3_2 into HepG2 cells, and genomic editing was verified by PCR. The donor nucleotide sequence in the final three codons of the MIER3 gene are modified to discourage sgRNA recognition, while retaining the native amino acid sequence. We performed ChIP-seq on this cell line using anti-FLAG Sigma F1804 and obtained data that passed our quality tests. Immunoprecipitation with anti-FLAG Sigma F1804 followed by mass spectrometry identified MIER3. All released data, including ChIP-seq files and validation data, are available at the ENCODE web portal: https://www.encodeproject.org/search/?searchTerm=flag&type=Experiment&lab.title=Richard+Myers%2C+HAIB . Note: This plasmid is part of the Mendenhall and Meyers CRISPR-based Tagging system to add a tag (currently using FLAG) to endogenous proteins. Please see https://www.addgene.org/crispr/tagging/ for more details.
growth strain :
Donor vector for 3' FLAG tag of human MIER3
origin :
37
pi :
|
resistance markers :
| 1728 |
| 2438 |
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
headquarters: USA
questions and comments