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product summary
company name :
Addgene
product type :
cDNA
product name :
pFETCh_HMG20A
catalog :
86271
citations: 1
product information
Catalog Number :
86271
Product Name :
pFETCh_HMG20A
article :
| doi | 10.1101/gr.193540.115 |
| id | 15802 |
| pubmed_id | 26355004 |
bacterial resistance :
Kanamycin
cloning :
| backbone | pFETCh_Donor | |
| backbone_mutation | ||
| backbone_origin | ||
| backbone_size | ||
| promoter | ||
| sequencing_primer_3 | ||
| sequencing_primer_5 | ||
| vector_types |
|
growth notes :
This plasmid was co-transfected along with PX458_HMG20A_1 and PX458_HMG20A_2 into HepG2 cells, and genomic editing was verified by PCR. The donor nucleotide sequence in the final codon of the HMG20A gene is modified to discourage sgRNA recognition, while retaining the native amino acid sequence. We performed ChIP-seq on this cell line using anti-FLAG Sigma F1804 and obtained data that passed our quality tests. Immunoprecipitation with anti-FLAG Sigma F1804 followed by mass spectrometry identified HMG20A. All released data, including ChIP-seq files and validation data, are available at the ENCODE web portal: https://www.encodeproject.org/search/?searchTerm=flag&type=Experiment&lab.title=Richard+Myers%2C+HAIB . Note: This plasmid is part of the Mendenhall and Meyers CRISPR-based Tagging system to add a tag (currently using FLAG) to endogenous proteins. Please see https://www.addgene.org/crispr/tagging/ for more details.
growth strain :
Donor vector for 3' FLAG tag of human HMG20A
origin :
37
pi :
|
resistance markers :
| 1728 |
| 2438 |
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
headquarters: USA
questions and comments