This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pDONOR-PARK6e5-I368N
catalog :
86154
citations: 1
Reference
Arias Fuenzalida J, Jarazo J, Qing X, Walter J, Gomez Giro G, Nickels S, et al. FACS-Assisted CRISPR-Cas9 Genome Editing Facilitates Parkinson's Disease Modeling. Stem Cell Reports. 2017;9:1423-1431 pubmed publisher
product information
Catalog Number :
86154
Product Name :
pDONOR-PARK6e5-I368N
article :
doi10.1016/j.stemcr.2017.08.026
id28192529
pubmed_id28988985
bacterial resistance :
Ampicillin
cloning :
backbonepUC-AMPr based
backbone_mutation
backbone_origin
backbone_size5888
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Bacterial Expression
growth strain :
Donor plasmid for PARK6 exon5 I368N sequence. Also contains TagBFP and dTomato
origin :
37
pi :
alt_names
PINK1
PARK6
BPRK
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3cgtcaattttacgcatgattatctttaac
sequencing_primer_5AGATGTCCTAAATGCACAGCG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesBRPK, PARK6
genePINK1
id65018
genbank_ids
NC_000001.11
NP_115785.1
mutation
namePINK1 exon 5 homology arms
shRNA_sequence
size5939
species
9606
Homo sapiens
tags
resistance markers :
3668
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA