pMXs2-MEK1 | Addgene 86141 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pMXs2-MEK1

catalog :

86141

citations: 1

Reference
Wang T, Yu H, Hughes N, Liu B, Kendirli A, Klein K, et al. Gene Essentiality Profiling Reveals Gene Networks and Synthetic Lethal Interactions with Oncogenic Ras. Cell. 2017;168:890-903.e15 pubmed publisher

product information

Catalog Number :

86141

Product Name :

pMXs2-MEK1

article :

doi	10.1016/j.cell.2017.01.013
id	25175
pubmed_id	28162770

bacterial resistance :

Ampicillin

cloning :

backbone

pMXs2-IRES-Bsd-p2A-RFP

backbone_mutation

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Retroviral

growth notes :

NOTE: GFP sequence present after the stop codon of the ORF that is not translated. A single nucleotide change at position 542 from G to T was identified resulting in a corresponding amino acid change at position 181 from Arg to Met.

growth strain :

Retroviral vector expressing MEK1-IRES-Bsd-p2A-RFP

origin :

pi :

alt_names

cloning

clone_method	Gibson Cloning
cloning_site_3
cloning_site_5
promoter	LTR
sequencing_primer_3
sequencing_primer_5	TACACGCCGCCCACGTGAAG
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	CFC3, MAPKK1, MEK1, MEL, MKK1, PRKMK1
gene	MAP2K1
id	5604

genbank_ids

mutation

name

MEK1

shRNA_sequence

size

species

9606

Homo sapiens