This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLenti-Tet-MKK6(DD)-Puro
catalog :
86094
citations: 2
Reference
Boyd R, Majumder S, Stiban J, Mavodza G, Straus A, Kempelingaiah S, et al. The heat shock protein Hsp27 controls mitochondrial function by modulating ceramide generation. Cell Rep. 2023;42:113081 pubmed publisher
Frank S, Berger P, Ljungman M, Miranti C. Human prostate luminal cell differentiation requires NOTCH3 induction by p38-MAPK and MYC. J Cell Sci. 2017;130:1952-1964 pubmed publisher
product information
Catalog Number :
86094
Product Name :
pLenti-Tet-MKK6(DD)-Puro
article :
doi10.1242/jcs.197152
id28189602
pubmed_id28446540
bacterial resistance :
Ampicillin
cloning :
backbonepLenti CMV/TO Puro DEST (670-1)
backbone_mutationL/R recombination to clone in MKK6-DD insert.
backbone_originEric Campeau
backbone_size8000
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth notes :
Note: The MKK6 insert in this plasmid has a S2A mutation compared to wild type, but the depositor has confirmed that it does not affect plasmid function. For proper use, cell line must first be engineered to express the Tetracycline Repressor (TetR). For example: Addgene plasmid #17492. (Not compatible with rtTA system) Source of MKK6-DD mutant construct: Alonso, G., Ambrosino, C., Jones, M. and Nebreda, A.R., 2000. Differential activation of p38 mitogen-activated protein kinase isoforms depending on signal strength. Journal of Biological Chemistry, 275(51), pp.40641-40648.
growth strain :
Tet/Dox inducible (TetR) constitutively active mutant MKK6/MAP2K6 in lentiviral vector
origin :
37
pi :
alt_names
MAP2K6
MEK6
MKK6-DD, constitutively active mutant
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoterCMV/TetO
sequencing_primer_3GCAGCGTATCCACATAGCG
sequencing_primer_5TGGGCGGTAGGCGTGTACG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesMAPKK6, MEK6, MKK6, PRKMK6, SAPKK-3, SAPKK3
geneMAP2K6
id5608
genbank_ids
NM_002758.3
mutationSerine 207 and Threonine 211 both changed to Aspartic Acid (D)
nameMKK6
shRNA_sequence
size1005
species
9606
Homo sapiens
tags
locationN terminal on insert
tagMyc tag
plasmid copy :
MKK6-DD gene construct originally made in the lab of Angel Nebreda. Citation in comments.
resistance markers :
3201
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA