This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCMV-SPORT6-hHMGCR1
catalog :
86085
citations: 2
Reference
Ho W, Chang J, Lim K, Cazenave Gassiot A, Nguyen A, Foo J, et al. TDP-43 mediates SREBF2-regulated gene expression required for oligodendrocyte myelination. J Cell Biol. 2021;220: pubmed publisher
Holic N, Frin S, Seye A, Galy A, Fenard D. Improvement of De Novo Cholesterol Biosynthesis Efficiently Promotes the Production of Human Immunodeficiency Virus Type 1-Derived Lentiviral Vectors. Hum Gene Ther Methods. 2017;28:67-77 pubmed publisher
product information
Catalog Number :
86085
Product Name :
pCMV-SPORT6-hHMGCR1
article :
doi
id22833
pubmed_id
bacterial resistance :
Ampicillin
cloning :
backbonepCMV-SPORT6-hHMGCR2
backbone_mutation
backbone_originI.M.A.G.E consortium (Library NIH_MGC_118, Image Id 5212903)
backbone_size5639
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
The pCMV-SPORT6-hHMGCR1 plasmid was obtained by inserting a fragment of human HMGCR1 in the pCMV-SPORT6-hHMGCR2 plasmid (Library NIH_MGC_118, Image Id 5212903) using BstBI and PvuII unique restriction sites. Addgene note: This plasmid also contains a portion of the 3' UTR of HMCGR1.
growth strain :
expresses the human HMG-CoA reductase isoform 1
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3PvuII
cloning_site_5BstBI
promoterCMV
sequencing_primer_3tcctttagaacccaatgccc
sequencing_primer_5ccggactctagcctaggccg
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesLDLCQ3, LGMDR28, MYPLG
geneHMGCR
id3156
genbank_ids
NM_000859.2
mutation
namehuman HMG-CoA Reductase isoform 1
shRNA_sequence
size2666
species
9606
Homo sapiens
tags
plasmid copy :
the I.M.A.G.E consortium (pCMV-SPORT6-hHMGCR2, Library NIH_MGC_118, Image Id 5212903),
resistance markers :
3189
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA