This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
GST-Rab8a-QL
catalog :
86079
citations: 2
Reference
Vides E, Adhikari A, Chiang C, Lis P, Purlyte E, Limouse C, et al. A feed-forward pathway drives LRRK2 kinase membrane recruitment and activation. elife. 2022;11: pubmed publisher
Madugula V, Lu L. A ternary complex comprising transportin1, Rab8 and the ciliary targeting signal directs proteins to ciliary membranes. J Cell Sci. 2016;129:3922-3934 pubmed
product information
Catalog Number :
86079
Product Name :
GST-Rab8a-QL
article :
doi10.1242/jcs.194019
id22774
pubmed_id27633000
bacterial resistance :
Ampicillin
cloning :
backbonepGEB
backbone_mutation
backbone_originLu Lei (J Cell Sci. 2001)
backbone_size5000
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth strain :
A bacterial expression plasmid encoding Rab8a GTPase-deficient mutant (Rab8a-Q67L) with N-terminus GST.
origin :
37
pi :
alt_names
ras-related protein Rab-8A
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5EcoRI
promotertac
sequencing_primer_35' GGAGCTGCATGTGTCAGAGG 3'
sequencing_primer_55' GGCAAGCCACGTTTGGTG 3'
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesMEL, RAB8
geneRAB8A
id4218
genbank_ids
NM_005370.4
mutationChanged glutamine 67 to leucine
namehRab8A
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on insert
tagGST
plasmid copy :
The original Rab8a clone was from Mammalian Gene Collection (IMAGE:3547214)
resistance markers :
3183
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA