This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
EGFP-Rab8a-TN
catalog :
86077
citations: 4
Reference
Miller M, McDermitt D, Sauvanet C, Lombardo A, Zaman R, Bretscher A. The RabGAPs EPI64A and EPI64B regulate the apical structure of epithelial cells †. Mol Biol Cell. 2022;33:ar8 pubmed publisher
Herbst S, Campbell P, Harvey J, Bernard E, Papayannopoulos V, Wood N, et al. LRRK2 activation controls the repair of damaged endomembranes in macrophages. EMBO J. 2020;:e104494 pubmed publisher
Liu Z, Bryant N, Kumaran R, Beilina A, Abeliovich A, Cookson M, et al. LRRK2 phosphorylates membrane-bound Rabs and is activated by GTP-bound Rab7L1 to promote recruitment to the trans-Golgi network. Hum Mol Genet. 2017;: pubmed publisher
Madugula V, Lu L. A ternary complex comprising transportin1, Rab8 and the ciliary targeting signal directs proteins to ciliary membranes. J Cell Sci. 2016;129:3922-3934 pubmed
product information
Catalog Number :
86077
Product Name :
EGFP-Rab8a-TN
article :
doi10.1242/jcs.194019
id22774
pubmed_id27633000
bacterial resistance :
Kanamycin
cloning :
backbonepEGFP-C2
backbone_mutation
backbone_originClontech
backbone_size4700
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
A mammalian expression plasmid encoding GDP locked Rab8a (Rab8a-T22N) with N-terminus EGFP.
origin :
37
pi :
alt_names
ras-related protein Rab-8A
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5EcoRI
promoterCMV
sequencing_primer_3
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesMEL, RAB8
geneRAB8A
id4218
genbank_ids
NM_005370.4
mutationChanged threonine 22 to aspargine
namehRab8A
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on insert
tagGFP
plasmid copy :
The original Rab8a clone was from Mammalian Gene Collection (IMAGE:3547214)
resistance markers :
3183
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA