This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
EGFP-Rab8a-wt
catalog :
86075
citations: 5
Reference
Raj N, Greune L, Kahms M, Mildner K, Franzkoch R, Psathaki O, et al. Early Endosomes Act as Local Exocytosis Hubs to Repair Endothelial Membrane Damage. Adv Sci (Weinh). 2023;10:e2300244 pubmed publisher
Wang J, Dang M, Day E. Inhibition of Wnt signaling by Frizzled7 antibody-coated nanoshells sensitizes triple-negative breast cancer cells to the autophagy regulator chloroquine. Nano Res. 2020;13:1693-1703 pubmed publisher
Herbst S, Campbell P, Harvey J, Bernard E, Papayannopoulos V, Wood N, et al. LRRK2 activation controls the repair of damaged endomembranes in macrophages. EMBO J. 2020;:e104494 pubmed publisher
Liu Z, Bryant N, Kumaran R, Beilina A, Abeliovich A, Cookson M, et al. LRRK2 phosphorylates membrane-bound Rabs and is activated by GTP-bound Rab7L1 to promote recruitment to the trans-Golgi network. Hum Mol Genet. 2017;: pubmed publisher
Madugula V, Lu L. A ternary complex comprising transportin1, Rab8 and the ciliary targeting signal directs proteins to ciliary membranes. J Cell Sci. 2016;129:3922-3934 pubmed
product information
Catalog Number :
86075
Product Name :
EGFP-Rab8a-wt
article :
doi10.1242/jcs.194019
id22774
pubmed_id27633000
bacterial resistance :
Kanamycin
cloning :
backbonepEGFP-C2
backbone_mutation
backbone_originClontech
backbone_size4700
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
A mammalian expression plasmid encoding Rab8a wild type with N-terminus EGFP.
origin :
37
pi :
alt_names
ras-related protein Rab-8A
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5EcoRI
promoterCMV
sequencing_primer_3
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesMEL, RAB8
geneRAB8A
id4218
genbank_ids
NM_005370.4
mutation
namehRab8A
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on insert
tagGFP
plasmid copy :
The original Rab8a clone was from Mammalian Gene Collection (IMAGE:3547214)
resistance markers :
3183
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA